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MARK3 antibody (AA 311-648)

This Rabbit Polyclonal antibody specifically detects MARK3 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN7601283

Quick Overview for MARK3 antibody (AA 311-648) (ABIN7601283)

Target

See all MARK3 Antibodies
MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

Reactivity

  • 59
  • 29
  • 19
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
Human, Mouse, Rat

Host

  • 50
  • 8
  • 1
Rabbit

Clonality

  • 51
  • 8
Polyclonal

Conjugate

  • 38
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MARK3 antibody is un-conjugated

Application

  • 36
  • 27
  • 21
  • 8
  • 6
  • 6
  • 3
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • Binding Specificity

    • 9
    • 8
    • 6
    • 6
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 311-648

    Purpose

    Anti-MARK3 Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-MARK3 Antibody Picoband® (ABIN7601283). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human MARK3 recombinant protein (Position: H311-D648).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Ansar, M., Chung, H., Waryah, Y. M., Makrythanasis, P., Falconnet, E., Rao, A. R., Guipponi, M., Narsani, A. K., Fingerhut, R., Santoni, F. A., Ranza, E., Waryah, A. M., Bellen, H. J., Antonarakis, S. E. Visual impairment and progressive phthisis bulbi caused by recessive pathogenic variant in MARK3. Hum. Molec. Genet. 27: 2703-2711, 2018. 2. Lennerz, J. K., Hurov, J. B., White, L. S., Lewandowski, K. T., Prior, J. L., Planer, G. J., Gereau, R. W., IV, Piwnica-Worms, D., Schmidt, R. E., Piwnica-Worms, H. Loss of Par-1a/MARK3/C-TAK1 kinase leads to reduced adiposity, resistance to hepatic steatosis, and defective gluconeogenesis. Molec. Cell. Biol. 30: 5043-5056, 2010. 3. Muller, J., Ory, S., Copeland, T., Piwnica-Worms, H., Morrison, D. K. C-TAK1 regulates Ras signaling by phosphorylating the MAPK scaffold, KSR1. Molec. Cell 8: 983-993, 2001.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

    Alternative Name

    MARK3

    Background

    Synonyms: Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG, CKAP5, KIAA0097

    Tissue Specificity: Expressed in fetal brain. Highly expressed in brain and placenta. Lower levels in heart, liver, thymus, kidney and lung. Located to endothelial cells and neuronal cells of the suprachiasmatic nucleus (SCN). Also detected in endothelial cells of the heart, lung and kidney. In the brain, specifically expressed in the thalamus, hippocampus and amygdala.

    Background: MAP/microtubule affinity-regulating kinase 3 is an enzyme that in humans is encoded by the MARK3 gene. The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene.

    Molecular Weight

    80 kDa

    Gene ID

    4140

    UniProt

    P27448

    Pathways

    SARS-CoV-2 Protein Interactome
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