MARK3 antibody (N-Term)

Catalog No. ABIN6258273

The Rabbit Polyclonal anti-MARK3 antibody is suitable to detect MARK3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and ICC.

Quick Overview for MARK3 antibody (N-Term) (ABIN6258273)

Target: MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MARK3 Antibody

Binding Specificity: N-Term

Specificity: MARK3 Antibody detects endogenous levels of total MARK3.

Predicted Reactivity: Pig,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MARK3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MARK3

Target: MARK3 (MAP/microtubule Affinity-Regulating Kinase 3 (MARK3))

Alternative Name: MARK3

Background:

Description: Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714).

Gene: MARK3

Molecular Weight: 85 kDa

Gene ID: 4140

UniProt: P27448

Pathways: SARS-CoV-2 Protein Interactome