MSI1 antibody (AA 33-347)

Catalog No. ABIN7601349

This Rabbit Polyclonal antibody specifically detects MSI1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for MSI1 antibody (AA 33-347) (ABIN7601349)

Target: MSI1 (Musashi Homolog 1 (Drosophila) (MSI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-MSI1 Antibody

Binding Specificity: AA 33-347

Purpose: Anti-Musashi 1/Msi1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Musashi 1/Msi1 Antibody Picoband® (ABIN7601349). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Musashi 1/Msi1 recombinant protein (Position: Q33-S347).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chavali, P. L., Stojic, L., Meredith, L. W., Joseph, N., Nahorski, M. S., Sanford, T. J., Sweeney, T. R., Krishna, B. A., Hosmillo, M., Firth, A. E., Bayliss, R., Marcelis, C. L., Lindsay, S., Goodfellow, I., Woods, C. G., Gergely, F. Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication. Science 357: 83-88, 2017. 2. Good, P., Yoda, A., Sakakibara, S., Yamamoto, A., Imai, T., Sawa, H., Ikeuchi, T., Tsuji, S., Satoh, H., Okano, H. The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells. Genomics 52: 382-384, 1998. 3. Siddall, N. A., McLaughlin, E. A., Marriner, N. L., Hime, G. R. The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity. Proc. Nat. Acad. Sci. 103: 8402-8407, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MSI1

Target: MSI1 (Musashi Homolog 1 (Drosophila) (MSI1))

Alternative Name: MSI1

Background:

Synonyms: RNA-binding protein Musashi homolog 1, Musashi-1, MSI1

Tissue Specificity: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

Background: RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.

Molecular Weight: 39 kDa

Gene ID: 4440

UniProt: O43347

Pathways: Stem Cell Maintenance