GLT25D1 antibody (AA 34-454)

Catalog No. ABIN7601391

This Rabbit Polyclonal antibody specifically detects GLT25D1 in ELISA, WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GLT25D1 antibody (AA 34-454) (ABIN7601391)

Target: GLT25D1 (Glycosyltransferase 25 Domain Containing 1 (GLT25D1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLT25D1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-GLT25D1 Antibody

Binding Specificity: AA 34-454

Purpose: Anti-GLT25D1/COLGALT1 Antibody Picoband®

Characteristics: Anti-GLT25D1/COLGALT1 Antibody Picoband® (ABIN7601391). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human COLGALT1 recombinant protein (Position: A34-D454). Human GLT25D1/COLGALT1 shares 93.6% amino acid (aa) sequence identity with mouse COLGALT1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Baumann, S., Hennet, T. Collagen accumulation in osteosarcoma cells lacking GLT25D1 collagen galactosyltransferase. J. Biol. Chem. 291: 18514-18524, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. June 9, 2017. 3. Liefhebber, J. M. P., Punt, S., Spaan, W. J. M., van Leeuwen, H. C. The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein. BMC Cell Biol. 11: 33, 2010. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GLT25D1

Target: GLT25D1 (Glycosyltransferase 25 Domain Containing 1 (GLT25D1))

Alternative Name: COLGALT1

Background: The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum.

Molecular Weight: 85 kDa

Gene ID: 79709

Pathways: SARS-CoV-2 Protein Interactome