SUFUH antibody (AA 34-484)

Catalog No. ABIN7601394

This Rabbit Polyclonal antibody specifically detects SUFUH in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SUFUH antibody (AA 34-484) (ABIN7601394)

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUFUH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SUFUH Antibody

Binding Specificity: AA 34-484

Purpose: Anti-SUFU Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SUFU Picoband® Antibody (ABIN7601394). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SUFU recombinant protein (Position: L34-H484).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aavikko, M., Li, S.-P., Saarinen, S., Alhopuro, P., Kaasinen, E., Morgunova, E., Li, Y., Vesanen, K., Smith, M. J., Evans, D. G. R., Poyhonen, M., Kiuru, A., Auvinen, A., Aaltonen, L. A., Taipale, J., Vahteristo, P. Loss of SUFU function in familial multiple meningioma. Am. J. Hum. Genet. 91: 520-526, 2012. 2. Brugieres, L., Pierron, G., Chompret, A., Bressac-de Paillerets, B., Di Rocco, F., Varlet, P., Pierre-Kahn, A., Caron, O., Grill, J., Delattre, O. Incomplete penetrance of the predisposition to medulloblastoma associated with germ-line SUFU mutations. J. Med. Genet. 47: 142-144, 2010. 3. De Mori, R., Romani, M., D'Arrigo, S., Zaki, M. S., Lorefice, E., Tardivo, S., Biagini, T., Stanley, V., Musaev, D., Fluss, J., Micalizzi, A., Nuovo, S., and 18 others. Hypomorphic recessive variants in SUFU impair the sonic hedgehog pathway and cause Joubert syndrome with cranio-facial and skeletal defects. Am. J. Hum. Genet. 101: 552-563, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SUFUH

Target: SUFUH (Suppressor of Fused Homolog (SUFUH))

Alternative Name: SUFU

Background:

Synonyms: Suppressor of fused homolog, SUFUH, SUFU, UNQ650, PRO1280

Tissue Specificity: Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle.

Background: Suppressor of fused homolog is a protein that in humans is encoded by the SUFU gene. It is mapped to 10q24.32. The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Molecular Weight: 54 kDa

Gene ID: 51684

Pathways: Hedgehog Signaling, Tube Formation, Maintenance of Protein Location