RLIM antibody (AA 369-621)

Catalog No. ABIN7601490

This Rabbit Polyclonal antibody specifically detects RLIM in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RLIM antibody (AA 369-621) (ABIN7601490)

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RLIM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-RLIM Antibody

Binding Specificity: AA 369-621

Purpose: Anti-RLIM Antibody Picoband®

Characteristics: Anti-RLIM Antibody Picoband® (ABIN7601490). Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RLIM recombinant protein (Position: R369-E621). Human RLIM shares 95.4% amino acid (aa) sequence identity with mouse RLIM.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bach, I., Rodriguez-Esteban, C., Carriere, C., Bhushan, A., Krones, A., Rose, D. W., Glass, C. K., Andersen, B., Belmonte, J. C. I., Rosenfeld, M. G. RLIM inhibits activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex. Nature Genet. 22: 394-399, 1999. 2. Frints, S. G. M., Ozanturk, A., Rodriguez Criado, G., Grasshoff, U., de Hoon, B., Field, M., Manouvrier-Hanu, S., Hickey, S. E., Kammoun, M., Gripp, K. W., Bauer, C., Schroeder, C., and 33 others. Pathogenic variants in E3 ubiquitin ligase RLIM/RNF12 lead to a syndromic X-linked intellectual disability and behavior disorder. Molec. Psychiat. 24: 1748-1768, 2019. 3. Gontan, C., Achame, E. M., Demmers, J., Barakat, T. S., Rentmeester, E., van IJcken, W., Grootegoed, J. A., Gribnau, J. RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation. Nature 485: 386-390, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RLIM

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Alternative Name: RLIM

Background: E3 ubiquitin-protein ligase RLIM is an enzyme that in humans is encoded by the RLIM gene. The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molecular Weight: 69 kDa

Gene ID: 51132