CENPH antibody (AA 37-247)

Catalog No. ABIN7601494

The Rabbit Polyclonal anti-CENPH antibody has been validated for WB, ELISA and FACS. It is suitable to detect CENPH in samples from Human.

Quick Overview for CENPH antibody (AA 37-247) (ABIN7601494)

Target: CENPH (Centromere Protein H (CENPH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CENPH Antibody

Binding Specificity: AA 37-247

Purpose: Anti-CENPH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CENPH Antibody Picoband® (ABIN7601494). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CENPH recombinant protein (Position: E37-M247).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Earnshaw, W. C. When is a centromere not a kinetochore? J. Cell Sci. 99: 1-4, 1991. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/4/2014. 3. Sugata, N., Li, S., Earnshaw, W. C., Yen, T. J., Yoda, K., Masumoto, H., Munekata, E., Warburton, P. E., Todokoro, K. Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes. Hum. Molec. Genet. 9: 2919-2926, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CENPH

Target: CENPH (Centromere Protein H (CENPH))

Alternative Name: CENPH

Background:

Synonyms: ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, HuB, Nervous system-specific RNA-binding protein Hel-N1, ELAVL2, HUB

Tissue Specificity: Brain, neural-specific.

Background: Centromere protein H is a protein that in humans is encoded by the CENPH gene. Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Molecular Weight: 28 kDa

Gene ID: 64946

UniProt: Q9H3R5