UBA5 antibody (AA 37-389)

Catalog No. ABIN7601503

The Rabbit Polyclonal anti-UBA5 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect UBA5 in samples from Human, Mouse and Rat.

Quick Overview for UBA5 antibody (AA 37-389) (ABIN7601503)

Target: UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-UBA5 Antibody

Binding Specificity: AA 37-389

Purpose: Anti-UBA5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-UBA5 Antibody Picoband® (ABIN7601503). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UBA5 recombinant protein (Position: R37-D389).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Colin, E., Daniel, J., Ziegler, A., Wakim, J., Scrivo, A., Haack, T. B., Khiati, S., Denomme, A.-S., Amati-Bonneau, P., Charif, M., Procaccio, V., Reynier, P., and 19 others. Biallelic variants in UBA5 reveal that disruption of the UFM1 cascade can result in early-onset encephalopathy. Am. J. Hum. Genet. 99: 695-703, 2016. 2. Dou, T., Gu, S., Liu, J., Chen, F., Zeng, L., Guo, L., Xie, Y., Mao, Y. Isolation and characterization of ubiquitin-activating enzyme E1-domain containing 1, UBE1DC1. Molec. Biol. Rep. 32: 265-271, 2005. 3. Duan, R., Shi, Y., Yu, L., Zhang, G., Li, J., Lin, Y., Guo, J., Wang, J., Shen, L., Jiang, H., Wang, G., Tang, B. UBA5 mutations cause a new form of autosomal recessive cerebellar ataxia. PLoS One 11: e0149039, 2016. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for UBA5

Target: UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))

Alternative Name: UBA5

Background:

Synonyms: Rho-related GTP-binding protein Rho6,Rho family GTPase 1,Rnd1,RND1,RHO6,

Tissue Specificity: Mostly expressed in brain and liver.

Background: Ubiquitin-like modifier-activating enzyme 5 is a protein that in humans is encoded by the UBA5 gene. This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1.

Molecular Weight: 50 kDa

Gene ID: 79876