Phospholipase D2 antibody (AA 37-924)

Catalog No. ABIN7601520

This Rabbit Polyclonal antibody specifically detects Phospholipase D2 in WB, ELISA, FACS, ICC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Phospholipase D2 antibody (AA 37-924) (ABIN7601520)

Target: Phospholipase D2 (PLD2)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Phospholipase D2 Antibody

Binding Specificity: AA 37-924

Purpose: Anti-PLD2 Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PLD2 Picoband® Antibody (ABIN7601520). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLD2 recombinant protein (Position: A37-E924).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Liscovitch, M., Czarny, M., Fiucci, G., Tang, X. Phospholipase D: molecular and cell biology of a novel gene family. Biochem. J. 345: 401-415, 2000. 2. Lopez, I., Arnold, R. S., Lambeth, J. D. Cloning and initial characterization of a human phospholipase D2 (hPLD2): ADP-ribosylation factor regulated hPLD2. J. Biol. Chem. 273: 12846-12852, 1998. 3. Park, S. H., Ryu, S. H., Suh, P. G., Kim, H. Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 82: 225 only, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Phospholipase D2

Target: Phospholipase D2 (PLD2)

Alternative Name: PLD2

Background:

Synonyms: Phospholipase D2, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2, PLD2

Tissue Specificity: Widely expressed.

Background: Phospholipase D2 is an enzyme that in humans is encoded by the PLD2 gene. It is mapped to 17p13.2. The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 106 kDa

Gene ID: 5338

UniProt: O14939

Pathways: RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling