POLR2H antibody (AA 38-150)

Catalog No. ABIN7601535

This Rabbit Polyclonal antibody specifically detects POLR2H in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for POLR2H antibody (AA 38-150) (ABIN7601535)

Target: POLR2H (Polymerase (RNA) II (DNA Directed) Polypeptide H (POLR2H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2H antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-POLR2H Antibody

Binding Specificity: AA 38-150

Purpose: Anti-POLR2H Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POLR2H Antibody Picoband® (ABIN7601535). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLR2H recombinant protein (Position: D38-F150).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. McKune, K., Moore, P. A., Hull, M. W., Woychik, N. A. Six human RNA polymerase subunits functionally substitute for their yeast counterparts. Molec. Cell. Biol. 15: 6895-6900, 1995. 2. Shpakovski, G. V., Acker, J., Wintzerith, M., Lacroix, J. F., Thuriaux, P., Vigneron, M. Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae. Molec. Cell. Biol. 15: 4702-4710, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLR2H

Target: POLR2H (Polymerase (RNA) II (DNA Directed) Polypeptide H (POLR2H))

Alternative Name: POLR2H

Background:

Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: DNA-ed RNA polymerases I, II, and III subunit RPABC3 is a protein that in humans is encoded by the POLR2H gene. The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-ed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.

Molecular Weight: 17 kDa

Gene ID: 5437

UniProt: P52434

Pathways: Regulatory RNA Pathways