Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (AA 38-401) antibody

Catalog No. ABIN7601543

The Rabbit Polyclonal anti-Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial antibody (ABIN7601543) specifically detects Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial in WB, IHC, ELISA, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (AA 38-401) antibody (ABIN7601543)

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 38-401

Purpose: Anti-ACADM/MCAD Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ACADM/MCAD Antibody (ABIN7601543). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACADM/MCAD recombinant protein (Position: S38-E401).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Alternative Name: ACADM

Background:

Synonyms: DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1, APPL1, APPL, DIP13A, KIAA1428

Tissue Specificity: High levels in heart, ovary, pancreas and skeletal muscle.

Background: ACADM (acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain) is a gene that provides instructions for making an enzyme called acyl-coenzyme A dehydrogenase that is important for breaking down (degrading) a certain group of fats called medium-chain fatty acids. This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 47 kDa

Gene ID: 34

UniProt: P11310