VPS51 antibody (AA 38-781)

Catalog No. ABIN7601551

Product Details anti-VPS51 Antibody

Target: VPS51 (Vacuolar Protein Sorting 51 Homolog (VPS51))

Binding Specificity: AA 38-781

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS51 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-FFR/VPS51 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FFR/VPS51 Antibody Picoband® (ABIN7601551). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FFR/VPS51 recombinant protein (Position: K38-R781).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gershlick, D. C., Ishida, M., Jones, J. R., Bellomo, A., Bonifacino, J. S., Everman, D. B. A neurodevelopmental disorder caused by mutations in the VPS51 subunit of the GARP and EARP complexes. Hum. Molec. Genet. 28: 1548-1560, 2019. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 4/14/2014. 3. Perez-Victoria, F. J., Schindler, C., Magadan, J. G., Mardones, G. A., Delevoye, C., Romao, M., Raposo, G., Bonifacino, J. S. Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex. Molec. Biol. Cell 21: 3386-3395, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for VPS51

Target: VPS51 (Vacuolar Protein Sorting 51 Homolog (VPS51))

Alternative Name: VPS51 (VPS51 Products)

Background:

Synonyms: PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80, PHF21A, BHC80, KIAA1696, BM-006

Tissue Specificity: Highly expressed in brain. Expressed at much lower level in other tissues.

Background: This gene encodes a member of the vacuolar protein sorting-associated protein 51 family. The encoded protein is a component of the Golgi-associated retrograde protein complex which acts as a tethering factor for carriers in retrograde transport from the early and late endosomes to the trans-Golgi network. Alternative splicing results in multiple transcript variants.

Molecular Weight: 86 kDa

Gene ID: 738