HECTD1 antibody (AA 39-566)

Catalog No. ABIN7601584

The Rabbit Polyclonal anti-HECTD1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect HECTD1 in samples from Human.

Quick Overview for HECTD1 antibody (AA 39-566) (ABIN7601584)

Target: HECTD1 (HECT Domain Containing 1 (HECTD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HECTD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-HECTD1 Antibody

Binding Specificity: AA 39-566

Purpose: Anti-HECTD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HECTD1 Antibody Picoband® (ABIN7601584). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HECTD1 recombinant protein (Position: D39-S566).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aleidi, S. M., Yang, A., Sharpe, L. J., Rao, G., Cochran, B. J., Rye, K.-A., Kockx, M., Brown, A. J., Gelissen, I. C. The E3 ubiquitin ligase, HECTD1, is involved in ABCA1-mediated cholesterol export from macrophages. Biochim. Biophys. Acta Molec. Cell Biol. Lipids 1863: 359-368, 2018. 2. D'Alonzo, D., Emch, F. H., Shen, X., Bruder, E., De Geyter, C., Zhang, H. Hectd1 is essential for embryogenesis in mice. Gene Expr. Patterns 34: 119064, 2019. Note: Electronic Article. 3. Gross, M. B. Personal Communication. Baltimore, Md. 10/29/2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HECTD1

Target: HECTD1 (HECT Domain Containing 1 (HECTD1))

Alternative Name: HECTD1

Background:

Synonyms: Kallistatin, Kallikrein inhibitor, Peptidase inhibitor 4, PI-4, Serpin A4, SERPINA4, KST, PI4

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.

Molecular Weight: 290 kDa

Gene ID: 25831

Pathways: Tube Formation, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection