DHX9 antibody (AA 396-759)

Catalog No. ABIN7601604

This Rabbit Polyclonal antibody specifically detects DHX9 in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DHX9 antibody (AA 396-759) (ABIN7601604)

Target: DHX9 (ATP-Dependent RNA Helicase A (DHX9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-DHX9 Antibody

Binding Specificity: AA 396-759

Purpose: Anti-RNA Helicase A/DHX9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for RNA Helicase A/DHX9 detection. Tested with WB, IHC, ICC/IF, IF, Flow Cytometry, ELISA in Human,Mouse,Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNA Helicase A/DHX9 recombinant protein (Position: E396-E759).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abdelhaleem, M. M., Hameed, S., Klassen, D., Greenberg, A. H. Leukophysin: an RNA helicase A-related molecule identified in cytotoxic T cell granules and vesicles. J. Immun. 156: 2026-2035, 1996. 2. Aktas, T., Avsar Ilik, I., Maticzka, D., Bhardwaj, V., Pessoa Rodrigues, C., Mittler, G., Manke, T., Backofen, R., Akhtar, A. DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Nature 544: 115-119, 2017. 3. Lee, C. G., Hurwitz, J. A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3-prime to 5-prime ion. J. Biol. Chem. 267: 4398-4407, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DHX9

Target: DHX9 (ATP-Dependent RNA Helicase A (DHX9))

Alternative Name: DHX9

Background:

Synonyms: ATP-dependent RNA helicase A, DEAH box protein 9, DExH-box helicase 9, Leukophysin, LKP, Nuclear DNA helicase II, NDH II, RNA helicase A, DHX9, DDX9

Tissue Specificity: Macrophages, peripheral blood leukocytes, lung, spleen and liver.

Background: ATP-dependent RNA helicase A (RHA, also known as DHX9, LKP, and NDHI) is an enzyme that in humans is encoded by the DHX9 gene. It is mapped to 1q25.3. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.

Molecular Weight: 141 kDa

Gene ID: 1660

UniProt: Q08211