ELK1 antibody (AA 4-52)

Catalog No. ABIN7601628

This Rabbit Polyclonal antibody specifically detects ELK1 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ELK1 antibody (AA 4-52) (ABIN7601628)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ELK1 Antibody

Binding Specificity: AA 4-52

Purpose: Anti-ELK1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ELK1 Antibody Picoband® (ABIN7601628). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ELK1 recombinant protein (Position: S4-K52).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barrett, L. E., Van Bockstaele, E. J., Sul, J. Y., Takano, H., Haydon, P. G., Eberwine, J. H. Elk-1 associates with the mitochondrial permeability transition pore complex in neurons. Proc. Nat. Acad. Sci. 103: 5155-5160, 2006. 2. Giovane, A., Sobieszczuk, P., Mignon, C., Mattei, M.-G., Wasylyk, B. Locations of the ets subfamily members net, elk1, and sap1 (ELK3, ELK1, and ELK4) on three homologous regions of the mouse and human genomes. Genomics 29: 769-772, 1995. 3. Janz, M., Lehmann, U., Olde Weghuis, D., de Leeuw, B., Geurts van Kessel, A., Gilgenkrantz, S., Hipskind, R. A., Nordheim, A. Refined mapping of the human Ets-related gene Elk-1 to Xp11.2-p11.4, distal to the OATL1 region. Hum. Genet. 94: 442-444, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: ELK1

Background:

Synonyms: Interleukin-6 receptor subunit alpha,IL-6 receptor subunit alpha,IL-6R subunit alpha,IL-6R-alpha,IL-6RA,IL-6R 1,Membrane glycoprotein 80,gp80,CD126,IL6R,

Tissue Specificity: Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

Background: ETS Like-1 protein Elk-1 is a protein that in humans is encoded by the ELK1. This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.

Molecular Weight: 50 kDa

Gene ID: 2002

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling