DBH antibody (AA 40-545)

Catalog No. ABIN7601651

This anti-DBH antibody is a Rabbit Polyclonal antibody detecting DBH in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for DBH antibody (AA 40-545) (ABIN7601651)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DBH Antibody

Binding Specificity: AA 40-545

Purpose: Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband® (ABIN7601651). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Dopamine beta Hydroxylase/DBH recombinant protein (Position: S40-N545).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Rush RA, Geffen LB (1980). "Dopamine beta-hydroxylase in health and disease". Critical Reviews in Clinical Laboratory Sciences. 12 (3): 241-77. 2. Kaufman S, Bridgers WF, Baron J (1968). "The Mechanism of Action of Dopamine beta-Hydroxylase". Advances in Chemistry. 77, chapter 73: 172-176. 3. Friedman S, Kaufman S (May 1966). "An electron paramagnetic resonance study of 3,4-dihydroxyphenylethylamine beta-hydroxylase". The Journal of Biological Chemistry. 241 (10): 2256-9.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Alternative Name: DBH

Background:

Synonyms: Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; DBH

Tissue Specificity: Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Background: Dopamine beta-hydroxylase  (DBH), also known as dopamine beta-monooxygenase, is an enzyme  (EC1.14.17.1) that in humans is encoded by the DBH gene. Dopamine beta-hydroxylase catalyzes the chemical reaction. It is mapped to 9q34.2. The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders.

Molecular Weight: 75 kDa

Gene ID: 1621

UniProt: P09172

Pathways: Carbohydrate Homeostasis