PRPF8 antibody (AA 433-2166)

Catalog No. ABIN7601730

This Rabbit Polyclonal antibody specifically detects PRPF8 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PRPF8 antibody (AA 433-2166) (ABIN7601730)

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRPF8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PRPF8 Antibody

Binding Specificity: AA 433-2166

Purpose: Anti-PRPF8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRPF8 Antibody Picoband® (ABIN7601730). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRPF8 recombinant protein (Position: E433-H2166).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Achsel, T., Ahrens, K., Brahms, H., Teigelkamp, S., Luhrmann, R. The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein. Molec. Cell. Biol. 18: 6756-6766, 1998. 2. Greenberg, J., Goliath, R., Beighton, P., Ramesar, R. A new locus for autosomal dominant retinitis pigmentosa on the short arm of chromosome 17. Hum. Molec. Genet. 3: 915-918, 1994. 3. Luo, H. R., Moreau, G. A., Levin, N., Moore, M. J. The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes. RNA 5: 893-908, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRPF8

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Alternative Name: PRPF8

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 13C, B-cell maturation defect, B-cell-activating factor receptor, BAFF receptor, BAFF-R, BLyS receptor 3, CD268, Tnfrsf13c, Baffr, Bcmd, Br3

Tissue Specificity: Highly expressed in spleen and testis, detected at lower levels in lung and thymus.

Background: Pre-mRNA-processing-splicing factor 8 is a protein that in humans is encoded by the PRPF8 gene. Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

Molecular Weight: 250 kDa

Gene ID: 10594

UniProt: Q6P2Q9