GRM2 antibody (AA 439-710)

Catalog No. ABIN7601733

This Rabbit Polyclonal antibody specifically detects GRM2 in ELISA, WB, IHC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for GRM2 antibody (AA 439-710) (ABIN7601733)

Target: GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GRM2 Antibody

Binding Specificity: AA 439-710

Purpose: Anti-Metabotropic glutamate receptor 2/GRM2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Metabotropic glutamate receptor 2/GRM2 Antibody (ABIN7601733). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Metabotropic glutamate receptor 2/GRM2 recombinant protein (Position: N439-A710).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Flor PJ, Lindauer K, Püttner I, Rüegg D, Lukic S, Knöpfel T, Kuhn R (April 1995). "Molecular cloning, functional expression and pharmacological characterization of the human metabotropic glutamate receptor type 2". The European Journal of Neuroscience. 7 (4): 622-9. 2. Kammermeier PJ, Davis MI, Ikeda SR (January 2003). "Specificity of metabotropic glutamate receptor 2 coupling to G proteins". Molecular Pharmacology. 63 (1): 183-91. 3. Jin LE, Wang M, Yang ST, Yang Y, Galvin VC, Lightbourne TC, Ottenheimer D, Zhong Q, Stein J, Raja A, Paspalas CD, Arnsten AF (November 2017). "mGluR2/3 mechanisms in primate dorsolateral prefrontal cortex: evidence for both presynaptic and postsynaptic actions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GRM2

Target: GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))

Alternative Name: GRM2

Background:

Synonyms: Metabotropic glutamate receptor 2; mGluR2; GRM2; GPRC1B, MGLUR2

Tissue Specificity: Detected in brain cortex (at protein level). Widely expressed in different regions of the adult brain as well as in fetal brain.

Background: Metabotropic glutamate receptor 2  (mGluR2) is a protein that, in humans, is encoded by the GRM2 gene. It is mapped to 3p21.2. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 100 kDa, >200 kDa

Gene ID: 2912

UniProt: Q14416

Pathways: cAMP Metabolic Process, Dicarboxylic Acid Transport