CA12 antibody (AA 45-298)

Catalog No. ABIN7601765

This anti-CA12 antibody is a Rabbit Polyclonal antibody detecting CA12 in WB and ELISA. Suitable for Human.

Quick Overview for CA12 antibody (AA 45-298) (ABIN7601765)

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CA12 Antibody

Binding Specificity: AA 45-298

Purpose: Anti-CA12 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CA12 Antibody Picoband® (ABIN7601765). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CA12 recombinant protein (Position: K45-A298).

Isotype: IgG

Application Details

Application Notes: "Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
"1. Hong, JH, Muhammad, E, Zheng, C, Hershkovitz, E, Alkrinawi, S, Loewenthal, N, Parvari, R, Muallem, S (15 December 2015). "Essential role of carbonic anhydrase XII in secretory gland fluid and HCO3 (-) secretion revealed by disease causing human mutation". The Journal of Physiology. 593 (24): 5299-312. 2. Lee, M, Vecchio-Pagán, B, Sharma, N, Waheed, A, Li, X, Raraigh, KS, Robbins, S, Han, ST, Franca, AL, Pellicore, MJ, Evans, TA, Arcara, KM, Nguyen, H, Luan, S, Belchis, D, Hertecant, J, Zabner, J, Sly, WS, Cutting, GR (23 February 2016). "Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease". Human Molecular Genetics. 25 (10): 1923-1933. 3. Almståhl, A, Wikström, M (May 2003). "Electrolytes in stimulated whole saliva in individuals with hyposalivation of different origins". Archives of Oral Biology. 48 (5): 337-44. doi:10.1016/s0003-9969(02)00200-5. PMID 12711377. Manokaran, S, Zhang, X, Chen, W, Srivastava, DK (June 2010). "Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine". Biochimica et Biophysica Acta. 1804 (6): 1376-84.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CA12

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: CA12

Background:

Synonyms: Carbonic anhydrase 12, Carbonate dehydratase XII, Carbonic anhydrase XII, CA-XII, Tumor antigen HOM-RCC-3.1.3, CA12

Tissue Specificity: Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).

Background: Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 15q22.2. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10 % of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.

Molecular Weight: 45 kDa

Gene ID: 771

UniProt: O43570