PRR5 antibody (AA 45-366)

Catalog No. ABIN7601773

The Rabbit Polyclonal anti-PRR5 antibody has been validated for WB, ELISA and FACS. It is suitable to detect PRR5 in samples from Human.

Quick Overview for PRR5 antibody (AA 45-366) (ABIN7601773)

Target: PRR5 (Proline Rich 5 (Renal) (PRR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PRR5 Antibody

Binding Specificity: AA 45-366

Purpose: Anti-PRR5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRR5 Antibody Picoband® (ABIN7601773). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRR5 recombinant protein (Position: S45-D366).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Johnstone, C. N., Castellvi-Bel, S., Chang, L. M., Sung, R. K., Bowser, M. J., Pique, J. M., Castells, A., Rustgi, A. K. PRR5 encodes a conserved proline-rich protein predominant in kidney: analysis of genomic organization, expression, and mutation status in breast and colorectal carcinomas. Genomics 85: 338-351, 2005. 2. Pearce, L. R., Huang, X., Boudeau, J., Pawlowski, R., Wullschleger, S., Deak, M., Ibrahim, A. F. M., Gourlay, R., Magnuson, M. A., Alessi, D. R. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem. J. 405: 513-522, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRR5

Target: PRR5 (Proline Rich 5 (Renal) (PRR5))

Alternative Name: PRR5

Background:

Synonyms: Long-chain-fatty-acid--CoA ligase 3,6.2.1.3,Long-chain acyl-CoA synthetase 3,LACS 3,ACSL3,ACS3, FACL3, LACS3,

Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Background: This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene.

Molecular Weight: 39 kDa

Gene ID: 55615

UniProt: P85299

Pathways: PI3K-Akt Signaling