GLI2 antibody (AA 46-1398)

Catalog No. ABIN7601790

This Rabbit Polyclonal antibody specifically detects GLI2 in WB, ELISA, IHC, IF, FACS and ICC. It exhibits reactivity toward Human and Rat.

Quick Overview for GLI2 antibody (AA 46-1398) (ABIN7601790)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-GLI2 Antibody

Binding Specificity: AA 46-1398

Purpose: Anti-GLI2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GLI2 Antibody Picoband® (ABIN7601790). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLI2 recombinant protein (Position: A46-A1398).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bertolacini, C. D. P., Ribeiro-Bicudo, L. A., Petrin, A., Richieri-Costa, A., Murray, J. C. Clinical findings in patients with GLI2 mutations--phenotypic variability. Clin. Genet. 81: 70-75, 2012. 2. Matsumoto, N., Fujimoto, M., Kato, R., Niikawa, N. Assignment of the human GLI2 gene to 2q14 by fluorescence in situ hybridization. Genomics 36: 220-221, 1996. 3. Roessler, E., Ermilov, A. N., Grange, D. K., Wang, A., Grachtchouk, M., Dlugosz, A. A., Muenke, M. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum. Molec. Genet. 14: 2181-2188, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2

Background:

Synonyms: Zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein, GLI2, THP

Tissue Specificity: Expressed in breast cancers. Isoform 1 and isoform 4 are expressed in HTLV-1-infected T-cell lines. Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines.

Background: GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes, thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. (1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. (2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

Molecular Weight: 180-200 kDa

Gene ID: 2736

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis