DBT antibody (AA 46-462)

Catalog No. ABIN7601800

The Rabbit Polyclonal anti-DBT antibody is suitable to detect DBT in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS, IF, ICC and IP.

Quick Overview for DBT antibody (AA 46-462) (ABIN7601800)

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-DBT Antibody

Binding Specificity: AA 46-462

Purpose: Anti-DBT Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.

Characteristics: Anti-DBT Antibody. Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DBT recombinant protein (Position: K46-R462). Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 2-4 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: DBT

Background:

Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Gene Full Name: dihydrolipoamide branched chain transacylase E2

Molecular Weight: 53 kDa

Gene ID: 1629

UniProt: P11182