GLB1 antibody (AA 46-655)

Catalog No. ABIN7601806

The Rabbit Polyclonal anti-GLB1 antibody has been validated for WB, IHC, ELISA, IF, ICC and FACS. It is suitable to detect GLB1 in samples from Human.

Quick Overview for GLB1 antibody (AA 46-655) (ABIN7601806)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GLB1 Antibody

Binding Specificity: AA 46-655

Purpose: Anti-GLB1/Beta-galactosidase Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GLB1/Beta-galactosidase Antibody Picoband® (ABIN7601806). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLB1/Beta-galactosidase recombinant protein (Position: Q46-K655).

Isotype: IgG

Application Details

Application Notes: "Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
"1. Shows TB, Scrafford-Wolff L, Brown JA, Meisler M (1978). "Assignment of a beta-galactosidase gene (beta GALA) to chromosome 3 in man". Cytogenetics and Cell Genetics. 22 (1-6): 219-22. 2. Oshima A, Tsuji A, Nagao Y, Sakuraba H, Suzuki Y (Nov 1988). "Cloning, sequencing, and expression of cDNA for human beta-galactosidase". Biochemical and Biophysical Research Communications. 157 (1): 238-44. 3. Caciotti A, Donati MA, Boneh A, d'Azzo A, Federico A, Parini R, Antuzzi D, Bardelli T, Nosi D, Kimonis V, Zammarchi E, Morrone A (Mar 2005). "Role of beta-galactosidase and elastin binding protein in lysosomal and nonlysosomal complexes of patients with GM1-gangliosidosis". Human Mutation. 25 (3): 285-92.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: GLB1

Background:

Synonyms: Beta-galactosidase, Acid beta-galactosidase, Lactase, Elastin receptor 1, GLB1, ELNR1

Tissue Specificity: Detected in placenta (at protein level) (PubMed:8383699). Detected in fibroblasts and testis (PubMed:2511208).

Background: Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. I t is mapped to 3p22.3. This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.

Molecular Weight: 65-85 kDa

Gene ID: 2720

UniProt: P16278

Pathways: Glycosaminoglycan Metabolic Process