HYOU1 antibody (AA 46-913)

Catalog No. ABIN7601807

This anti-HYOU1 antibody is a Rabbit Polyclonal antibody detecting HYOU1 in WB, IHC, IF, ELISA and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for HYOU1 antibody (AA 46-913) (ABIN7601807)

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HYOU1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-HYOU1 Antibody

Binding Specificity: AA 46-913

Purpose: Anti-ORP150/HYOU1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-O/HYOU1 Antibody Picoband® (ABIN7601807). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ORP150/HYOU1 recombinant protein (Position: K46-K913).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Haapaniemi, E. M., Fogarty, C. L., Keskitalo, S., Katayama, S., Vihinen, H., Ilander, M., Mustjoki, S., Krjutskov, K., Lehto, M., Hautala, T., Eriksson, O., Jokitalo, E., Velagapudi, V., Varjosalo, M., Seppanen, M., Kere, J. Combined immunodeficiency and hypoglycemia associated with mutations in hypoxia upregulated 1. (Letter) J. Allergy Clin. Immun. 139: 1391-1393, 2017. 2. Ikeda, J., Kaneda, S., Kuwabara, K., Ogawa, S., Kobayashi, T., Matsumoto, M., Yura, T., Yanagi, H. Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150. Biochem. Biophys. Res. Commun. 230: 94-99, 1997. 3. Janeczko, K. The proliferative response of S-100 protein-positive glial cells to injury in the neonatal rat brain. Brain Res. 564: 86-90, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: HYOU1

Background:

Synonyms: Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8, REC8L1

Tissue Specificity: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.

Background: Hypoxia up-regulated protein 1 is a protein that in humans is encoded by the HYOU1 gene. The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants.

Molecular Weight: 150 kDa

Gene ID: 10525

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome