CLIC1 antibody (AA 47-241)

Catalog No. ABIN7601812

The Rabbit Polyclonal anti-CLIC1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect CLIC1 in samples from Human, Rat and Monkey.

Quick Overview for CLIC1 antibody (AA 47-241) (ABIN7601812)

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLIC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CLIC1 Antibody

Binding Specificity: AA 47-241

Purpose: Anti-CLIC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CLIC1 Antibody Picoband® (ABIN7601812). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CLIC1 recombinant protein (Position: D47-K241).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat, Monkey
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Singh, H., Cousin, M. A., Ashley, R. H. Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin. FEBS J. 274: 6306-6316, 2007. 2. Valenzuela, S. M., Martin, D. K., Por, S. B., Robbins, J. M., Warton, K., Bootcov, M. R., Schofield, P. R., Campbell, T. J., Breit, S. N. Molecular cloning and expression of a chloride ion channel of cell nuclei. J. Biol. Chem. 272: 12575-12582, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CLIC1

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Alternative Name: CLIC1

Background:

Synonyms: Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12, CASP2, ICH1, NEDD2

Tissue Specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Background: Chloride intracellular channel protein 1 is a protein that in humans is encoded by the CLIC1 gene. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH , and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family, the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.

Molecular Weight: 32 kDa

Gene ID: 1192

UniProt: O00299