SMO antibody (AA 476-764)

Catalog No. ABIN7601825

This anti-SMO antibody is a Rabbit Polyclonal antibody detecting SMO in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for SMO antibody (AA 476-764) (ABIN7601825)

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SMO Antibody

Binding Specificity: AA 476-764

Purpose: Anti-Smoothened/SMO Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Smoothened/SMO Antibody (ABIN7601825). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Smoothened/SMO recombinant protein (Position: N476-Q764).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SMO

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Alternative Name: SMO

Background:

Synonyms: Peroxisome proliferator-activated receptor gamma,PPAR-gamma,Nuclear receptor subfamily 1 group C member 3,PPARG,NR1C3,

Tissue Specificity: Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. .

Background: Smoothened is a protein that in humans is encoded by the SMO gene. The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Molecular Weight: 86 kDa

Gene ID: 6608

UniProt: Q99835

Pathways: Hedgehog Signaling