SMO antibody (AA 476-764)

Catalog No. ABIN7601825

This anti-SMO antibody is a Rabbit Polyclonal antibody detecting SMO in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for SMO antibody (AA 476-764) (ABIN7601825)

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SMO Antibody

Binding Specificity: AA 476-764

Purpose: Anti-Smoothened/SMO Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Smoothened/SMO Antibody Picoband® (ABIN7601825). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Smoothened/SMO recombinant protein (Position: N476-Q764).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Byrne, E. F. X., Sircar, R., Miller, P. S., Hedger, G., Luchetti, G., Nachtergaele, S., Tully, M. D., Mydock-McGrane, L., Covey, D. F., Rambo, R. P., Sansom, M. S. P., Newstead, S., Rohatgi, R., Siebold, C. Structural basis of Smoothened regulation by its extracellular domains. Nature 535: 517-522, 2016. 2. Chen, W., Ren, X.-R., Nelson, C. D., Barak, L. S., Chen, J. K., Beachy, P. A., de Sauvage, F., Lefkowitz, R. J. Activity-dependent internalization of Smoothened mediated by beta-arrestin 2 and GRK2. Science 306: 2257-2260, 2004. 3. Chen, Y., Struhl, G. Dual roles for Patched in sequestering and transducing hedgehog. Cell 87: 553-563, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SMO

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Alternative Name: SMO

Background:

Synonyms: Peroxisome proliferator-activated receptor gamma,PPAR-gamma,Nuclear receptor subfamily 1 group C member 3,PPARG,NR1C3,

Tissue Specificity: Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. .

Background: Smoothened is a protein that in humans is encoded by the SMO gene. The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Molecular Weight: 86 kDa

Gene ID: 6608

UniProt: Q99835

Pathways: Hedgehog Signaling