ITK antibody (AA 49-465)

Catalog No. ABIN7601855

This anti-ITK antibody is a Rabbit Polyclonal antibody detecting ITK in WB, ELISA, IHC and FACS. Suitable for Human.

Quick Overview for ITK antibody (AA 49-465) (ABIN7601855)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-ITK Antibody

Binding Specificity: AA 49-465

Purpose: Anti-ITK Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ITK Antibody Picoband® (ABIN7601855). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ITK recombinant protein (Position: R49-F465).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: ITK IL2-inducible T-cell kinase". 2. Gibson S, Leung B, Squire JA, Hill M, Arima N, Goss P, Hogg D, Mills GB (September 1993)."Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q". Blood. 82 (5): 1561-72. 3. Gomez-Rodriguez J, Kraus ZJ, Schwartzberg PL (March 2011). "Tec family kinases Itk and Rlk/Txk in T lymphocytes: cross-regulation of cytokine production and T-cell fates.". FEBS.278 (12): 1980-1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK

Background:

Synonyms: Sphingosine kinase 2, SK 2, SPK 2

Background: Tyrosine-protein kinase ITK/TSK, also known as interleukin-2-inducible T-cell kinase or simply ITK, is a protein that in humans is encoded by the ITK gene. It is a member of the TEC family of kinases. This gene is mapped to 5q33.3. This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein is thought to play a role in T-cell proliferation and differentiation. Furthermore, ITK is functionally important for the development and effector function of Th2 and Th17 cells.

Molecular Weight: 72 kDa

Gene ID: 3702

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway