AFAP antibody (AA 498-730)

Catalog No. ABIN7601861

This anti-AFAP antibody is a Rabbit Polyclonal antibody detecting AFAP in WB, ELISA, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for AFAP antibody (AA 498-730) (ABIN7601861)

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-AFAP Antibody

Binding Specificity: AA 498-730

Purpose: Anti-AFAP/AFAP1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AFAP/AFAP1 Antibody (ABIN7601861). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AFAP/AFAP1 recombinant protein (Position: A498-T730).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL,Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AFAP

Target: AFAP (AFAP1) (Actin Filament Associated Protein 1 (AFAP1))

Alternative Name: AFAP1

Background:

Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).

Background: Actin filament-associated protein 1 is a protein that in humans is encoded by the AFAP1 gene. The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 120 kDa

Gene ID: 60312

UniProt: Q8N556