SCYL1 antibody (AA 5-259)

Catalog No. ABIN7601875

The Rabbit Polyclonal anti-SCYL1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect SCYL1 in samples from Human.

Quick Overview for SCYL1 antibody (AA 5-259) (ABIN7601875)

Target: SCYL1 (SCY1-Like 1 (SCYL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCYL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SCYL1 Antibody

Binding Specificity: AA 5-259

Purpose: Anti-SCYL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SCYL1 Antibody Picoband® (ABIN7601875). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SCYL1 recombinant protein (Position: A5-Q259).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Di, Y., Li, J., Fang, J., Xu, Z., He, X., Zhang, F., Ling, J., Li, X., Xu, D., Li, L., Li, Y.-Y., Huo, K. Cloning and characterization of a novel gene which encodes a protein interacting with the mitosis-associated kinase-like protein NTKL. J. Hum. Genet. 48: 315-321, 2003. 2. Kato, M., Yano, K., Morotomi-Yano, K., Saito, H., Miki, Y. Identification and characterization of the human protein kinase-like gene NTKL: mitosis-specific centrosomal localization of an alternatively spliced isoform. Genomics 79: 760-767, 2002. 3. Lenz, D., McClean, P., Kansu, A., Bonnen, P. E., Ranucci, G., Thiel, C., Straub, B. K., Harting, I., Alhaddad, B., Dimitrov, B., Kotzaeridou, U., Wenning, D., and 11 others. SCYL1 variants cause a syndrome with low gamma-glutamyl-transferase cholestasis, acute liver failure, and neurodegeneration (CALFAN). Genet. Med. 20: 1255-1265, 2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SCYL1

Target: SCYL1 (SCY1-Like 1 (SCYL1))

Alternative Name: SCYL1

Background:

Synonyms: C-C chemokine receptor type 10, C-C CKR-10, CC-CKR-10, CCR-10, G-protein coupled receptor 2, CCR10, GPR2

Tissue Specificity: Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.

Background: SCY1-like 1 (S. cerevisiae), also known as SCYL1, is a human gene which is highly conserved throughout evolution. This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 115 kDa

Gene ID: 57410

Pathways: p53 Signaling