AMPD1 antibody (AA 5-747)

Catalog No. ABIN7601893

The Rabbit Polyclonal anti-AMPD1 antibody has been validated for ELISA, WB, IHC and FACS. It is suitable to detect AMPD1 in samples from Human, Mouse and Rat.

Quick Overview for AMPD1 antibody (AA 5-747) (ABIN7601893)

Target: AMPD1 (Adenosine Monophosphate Deaminase 1 (AMPD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMPD1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-AMPD1 Antibody

Binding Specificity: AA 5-747

Purpose: Anti-AMPD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-AMPD1 Antibody Picoband® (ABIN7601893). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AMPD1 recombinant protein (Position: K5-E747). Human AMPD1 shares 92.7% amino acid (aa) sequence identity with both mouse and rat AMPD1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abe, M., Higuchi, I., Morisaki, H., Morisaki, T., Osame, M. Myoadenylate deaminase deficiency with progressive muscle weakness and atrophy caused by new missense mutations in AMPD1 gene: case report in a Japanese patient. Neuromusc. Disord. 10: 472-477, 2000. 2. Castro-Gago, M., Gomez-Lado, C., Perez-Gay, L., Eiris-Punal, J., Martinez, E. P., Garcia-Consuegra, I., Martin, M. A. Primary adenosine monophosphate (AMP) deaminase deficiency in a hypotonic infant. J. Child Neurol. 26: 734-737, 2011. 3. Genetta, T., Morisaki, H., Morisaki, T., Holmes, E. W. A novel bipartite intronic splicing enhancer promotes the inclusion of a mini-exon in the AMP deaminase 1 gene. J. Biol. Chem. 276: 25589-25597, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for AMPD1

Target: AMPD1 (Adenosine Monophosphate Deaminase 1 (AMPD1))

Alternative Name: AMPD1

Background:

Synonyms: AMPD1, AMP deaminase 1, EC 3.5.4.6, AMP deaminase isoform M, Myoadenylate deaminase

Background: AMP deaminase 1 is an enzyme that in humans is encoded by the AMPD1 gene. Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Molecular Weight: 86 kDa

Gene ID: 270

UniProt: P23109