SPDEF/PSE antibody (AA 52-335)

Catalog No. ABIN7601954

This anti-SPDEF/PSE antibody is a Rabbit Polyclonal antibody detecting SPDEF/PSE in WB, ELISA, FACS, IF and ICC. Suitable for Human.

Quick Overview for SPDEF/PSE antibody (AA 52-335) (ABIN7601954)

Target: SPDEF/PSE (SPDEF) (Proximal Sequence Element 1 (SPDEF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPDEF/PSE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SPDEF/PSE Antibody

Binding Specificity: AA 52-335

Purpose: Anti-PSE/SPDEF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PSE/SPDEF Antibody Picoband® (ABIN7601954). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PSE/SPDEF recombinant protein (Position: E52-I335).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Oettgen, P., Finger, E., Sun, Z., Akbarali, Y., Thamrongsak, U., Boltax, J., Grall, F., Dube, A., Weiss, A., Brown, L., Quinn, G., Kas, K., Endress, G., Kunsch, C., Libermann, T. A. PDEF, a novel prostate epithelium-specific Ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression. J. Biol. Chem. 275: 1216-1225, 2000. 2. Park, K.-S., Korfhagen, T. R., Bruno, M. D., Kitzmiller, J. A., Wan, H., Wert, S. E., Hershey, G. K. K., Chen, G., Whitsett, J. A. SPDEF regulates goblet cell hyperplasia in the airway epithelium. J. Clin. Invest. 117: 978-988, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SPDEF/PSE

Target: SPDEF/PSE (SPDEF) (Proximal Sequence Element 1 (SPDEF))

Alternative Name: SPDEF

Background:

Synonyms: Type I iodothyronine deiodinase, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: SAM pointed domain-containing Ets transcription factor is a protein that in humans is encoded by the SPDEF gene. The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 44 kDa

Gene ID: 25803

UniProt: O95238