DHPS antibody (AA 54-358)

Catalog No. ABIN7601998

The Rabbit Polyclonal anti-DHPS antibody has been validated for WB, ELISA, IHC, ICC, IF and FACS. It is suitable to detect DHPS in samples from Human, Mouse and Rat.

Quick Overview for DHPS antibody (AA 54-358) (ABIN7601998)

Target: DHPS (Deoxyhypusine Synthase (DHPS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHPS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-DHPS Antibody

Binding Specificity: AA 54-358

Purpose: Anti-DHPS/DHS Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DHPS/DHS Antibody Picoband® (ABIN7601998). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DHPS recombinant protein (Position: F54-K358).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bevec, D., Kappel, B., Jaksche, H., Csonga, R., Hauber, J., Klier, H., Steinkasserer, A. Molecular characterization of a cDNA encoding functional human deoxyhypusine synthase and chromosomal mapping of the corresponding gene locus. FEBS Lett. 378: 195-198, 1996. 2. Ganapathi, M., Padgett, L. R., Yamada, K., Devinsky, O., Willaert, R., Person, R., Au, P.-Y. B., Tagoe, J., McDonald, M., Karlowicz, D., Wolf, B., Lee, J., and 10 others. Recessive rare variants in deoxyhypusine synthase, an enzyme involved in the synthesis of hypusine, are associated with a neurodevelopmental disorder. Am. J. Hum. Genet. 104: 287-298, 2019. 3. Hauber, I., Bevec, D., Heukeshoven, J., Kratzer, F., Horn, F., Choidas, A., Harrer, T., Hauber, J. Identification of cellular deoxyhypusine synthase as a novel target for antiretroviral therapy. J. Clin. Invest. 115: 76-85, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DHPS

Target: DHPS (Deoxyhypusine Synthase (DHPS))

Alternative Name: DHPS

Background:

Synonyms: Beta-3 adrenergic receptor, Beta-3 adrenoreceptor, Beta-3 adrenoceptor, ADRB3, ADRB3R, B3AR

Tissue Specificity: Expressed mainly in adipose tissues.

Background: Deoxyhypusine synthase is an enzyme that in humans is encoded by the DHPS gene. This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 41 kDa

Gene ID: 1725

UniProt: P49366