ZNRF3 antibody (AA 54-913)

Catalog No. ABIN7602004

The Rabbit Polyclonal anti-ZNRF3 antibody has been validated for ELISA, WB, IF, FACS and ICC. It is suitable to detect ZNRF3 in samples from Human.

Quick Overview for ZNRF3 antibody (AA 54-913) (ABIN7602004)

Target: ZNRF3 (Zinc and Ring Finger Protein 3 (ZNRF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNRF3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ZNRF3 Antibody

Binding Specificity: AA 54-913

Purpose: Anti-ZNRF3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ZNRF3 Antibody Picoband® (ABIN7602004). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ZNRF3 recombinant protein (Position: R54-E913).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hao, H.-X., Xie, Y., Zhang, Y., Charlat, O., Oster, E., Avello, M., Lei, H., Mickanin, C., Liu, D., Ruffner, H., Mao, X., Ma, Q., Zamponi, R., Bouwmeester, T., Finan, P. M., Kirschner, M. W., Porter, J. A., Serluca, F. C., Cong, F. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature 485: 195-200, 2012. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/16/2008. 3. Hirosawa, M., Nagase, T., Ishikawa, K., Kikuno, R., Nomura, N., Ohara, O. Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. DNA Res. 6: 329-336, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ZNRF3

Target: ZNRF3 (Zinc and Ring Finger Protein 3 (ZNRF3))

Alternative Name: ZNRF3

Background:

Synonyms: Cerebellar degeneration-related protein 2, Major Yo paraneoplastic antigen, Paraneoplastic cerebellar degeneration-associated antigen, CDR2, PCD17

Tissue Specificity: Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.

Background: Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in cellular protein metabolic process and negative regulation of Wnt signaling pathway. Is integral component of plasma membrane.

Molecular Weight: 130 kDa

Gene ID: 84133

Pathways: WNT Signaling