OS9 antibody (AA 56-635)

Catalog No. ABIN7602048

This Rabbit Polyclonal antibody specifically detects OS9 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for OS9 antibody (AA 56-635) (ABIN7602048)

Target: OS9 (Osteosarcoma Amplified 9, Endoplasmic Reticulum Lectin (OS9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OS9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-OS9 Antibody

Binding Specificity: AA 56-635

Purpose: Anti-OS9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-OS9 Antibody Picoband® (ABIN7602048). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human OS9 recombinant protein (Position: Q56-E635).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Baek, J. H., Mahon, P. C., Oh, J., Kelly, B., Krishnamachary, B., Pearson, M., Chan, D. A., Giaccia, A. J., Semenza, G. L. OS-9 interacts with hypoxia-inducible factor 1-alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1-alpha. Molec. Cell 17: 503-512, 2005. 2. Bhamidipati, A., Denic, V., Quan, E. M., Weissman, J. S. Exploration of the topological requirements of ERAD identifies Yos9p as a lectin sensor of misfolded glycoproteins in the ER lumen. Molec. Cell 19: 741-751, 2005. 3. Friedmann, E., Salzberg, Y., Weinberger, A., Shaltiel, S., Gerst, J. E. YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins. J. Biol. Chem. 277: 35274-35281, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for OS9

Target: OS9 (Osteosarcoma Amplified 9, Endoplasmic Reticulum Lectin (OS9))

Alternative Name: OS9

Background:

Synonyms: Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Erf1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1

Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Background: Protein OS-9 is a protein that in humans is encoded by the OS9 gene. This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 83 kDa

Gene ID: 10956

UniProt: Q13438

Pathways: Maintenance of Protein Location, SARS-CoV-2 Protein Interactome