MCAM antibody (AA 59-401)

Catalog No. ABIN7602097

The Mouse Monoclonal anti-MCAM antibody is suitable to detect MCAM in samples from Human. It has been validated for IHC, WB and IHC (fro).

Quick Overview for MCAM antibody (AA 59-401) (ABIN7602097)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2H12

Product Details anti-MCAM Antibody

Binding Specificity: AA 59-401

Purpose: Anti-CD146/MCAM Antibody (monoclonal, 2H12)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human CD146 shares 73% amino acid (aa) sequence identity with both mouse,rat CD146.

Characteristics: Anti-CD146/MCAM Antibody.(monoclonal, 2H12)-4. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM

Background:

Background: CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113 kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.

Gene Full Name: melanoma cell adhesion molecule

Molecular Weight: 120 kDa

Gene ID: 4162

UniProt: P43121