EPH Receptor A3 antibody (AA 59-514)

Catalog No. ABIN7602102

This Rabbit Polyclonal antibody specifically detects EPH Receptor A3 in WB, ELISA, IHC, FACS, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for EPH Receptor A3 antibody (AA 59-514) (ABIN7602102)

Target: EPH Receptor A3 (EPHA3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EPH Receptor A3 Antibody

Binding Specificity: AA 59-514

Purpose: Anti-Eph receptor A3/EPHA3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Eph receptor A3/EPHA3 Antibody Picoband® (ABIN7602102). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Eph receptor A3/EPHA3 recombinant protein (Position: D59-A514).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Boyd, A. W., Ward, L. D., Wicks, I. P., Simpson, R. J., Salvaris, E., Wilks, A., Welch, K., Loudovaris, M., Rockman, S., Busmanis, I. Isolation and characterization of a novel receptor-type protein tyrosine kinase (hek) from a human pre-B cell line. J. Biol. Chem. 267: 3262-3267, 1992. 2. Fox, G. M., Holst, P. L., Chute, H. T., Lindberg, R. A., Janssen, A. M., Basu, R., Welcher, A. A. cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases. Oncogene 10: 897-905, 1995. 3. Gallarda, B. W., Bonanomi, D., Mueller, D., Brown, A., Alaynick, W. A., Andrews S. E., Lemke, G., Pfaff, S. L., Marquardt, T. Segregation of axial motor and sensory pathways via heterotypic trans-axonal signaling. Science 320: 233-236, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for EPH Receptor A3

Target: EPH Receptor A3 (EPHA3)

Alternative Name: EPHA3

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 13C, B-cell maturation defect, B-cell-activating factor receptor, BAFF receptor, BAFF-R, BLyS receptor 3, CD268, Tnfrsf13c, Baffr, Bcmd, Br3

Tissue Specificity: Highly expressed in spleen and testis, detected at lower levels in lung and thymus.

Background: Ephrin Receptor EphA3, is also known as human embryo kinase(HEK) or Eph-like tyrosine kinase1(ETK1). Kinases that phosphorylate proteins on tyrosine residues(protein tyrosine kinases, PTKs), such as EPHA3, form a structurally related group of molecules that exhibit functional diversity. Genetic alterations that lead to the inappropriate activation or expression of PTKs may be oncogenic. Many growth factor receptors are PTKs, e.g., the receptors for epidermal growth factor(EGFR), platelet-derived growth factor(PDGFR1,PDGFR2), colony-stimulating factor-1(CSF1R), and stem cell growth factor. The EPHA3 gene is mapped to 3p11.2. Sequence comparison with other PTKs showed a high degree of homology with members of the EPH and ELK(EPHB1) families of receptor tyrosine kinases. Within axial nerves, establishment of discrete afferent and efferent pathways depends on coordinate signaling between coextending sensory and motor projections.

Molecular Weight: 135 kDa

Gene ID: 2042

UniProt: P29320

Pathways: RTK Signaling, Regulation of Cell Size