Aryl Hydrocarbon Receptor antibody (AA 6-842)

Catalog No. ABIN7602143

This anti-Aryl Hydrocarbon Receptor antibody is a Rabbit Polyclonal antibody detecting Aryl Hydrocarbon Receptor in WB, IHC, ELISA, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Aryl Hydrocarbon Receptor antibody (AA 6-842) (ABIN7602143)

Target: Aryl Hydrocarbon Receptor (AHR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aryl Hydrocarbon Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Aryl Hydrocarbon Receptor Antibody

Binding Specificity: AA 6-842

Purpose: Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Aryl hydrocarbon Receptor/AHR Antibody Picoband® (ABIN7602143). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Aryl hydrocarbon Receptor/AHR recombinant protein (Position: A6-L842).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Le Beau, M. M., Carver, L. A., Espinosa, R., III, Schmidt, J. V., Bradfield, C. A. Chromosomal localization of the human AHR locus encoding the structural gene for the Ah receptor to 7p21-p15. Cytogenet. Cell Genet. 66: 172-176, 1994. 2. Rentas, S., Holzapfel, N. T., Belew, M. S., Pratt, G. A., Voisin, V., Wilhelm, B. T., Bader, G. D., Yeo, G. W., Hope, K. J. Musashi-2 attenuates AHR signalling to expand human haematopoietic stem cells. Nature 532: 508-511, 2016. 3. Schiering, C., Wincent, E., Metidji, A., Iseppon, A., Li, Y., Potocnik, A. J., Omenetti, S., Henderson, C. J., Wolf, C. R., Nebert, D. W., Stockinger, B. Feedback control of AHR signalling regulates intestinal immunity. Nature 542: 242-245, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Aryl Hydrocarbon Receptor

Target: Aryl Hydrocarbon Receptor (AHR)

Alternative Name: AHR

Background:

Synonyms: Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76, AHR, BHLHE76

Tissue Specificity: Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. Expressed in retinal photoreceptors.

Background: The aryl hydrocarbon receptor (AhR or AHR or ahr or ahR) is a protein that in humans is encoded by the AHR gene. It is mapped to 7p21.1. The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm, upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes.

Molecular Weight: 96 kDa

Gene ID: 196

UniProt: P35869

Pathways: Regulation of Cell Size