CARD10 antibody (AA 63-1014)

Catalog No. ABIN7602201

This Rabbit Polyclonal antibody specifically detects CARD10 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CARD10 antibody (AA 63-1014) (ABIN7602201)

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-CARD10 Antibody

Binding Specificity: AA 63-1014

Purpose: Anti-CARD10 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CARD10 Antibody Picoband® (ABIN7602201). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CARD10 recombinant protein (Position: E63-R1014). Human CARD10 shares 90.7% amino acid (aa) sequence identity with mouse CARD10.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Gaide, O., Martinon, F., Micheau, O., Bonnet, D., Thome, M., Tschopp, J. Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappa-B activation. FEBS Lett. 496: 121-127, 2001. 2. Grabiner, B. C., Blonska, M., Lin, P.-C., You, Y., Wang, D., Sun, J., Darnay, B. G., Dong, C., Lin, X. CARMA3 deficiency abrogates G protein-coupled receptor-induced NF-kappa-B activation. Genes Dev. 21: 984-996, 2007. 3. McAllister-Lucas, L. M., Inohara, N., Lucas, P. C., Ruland, J., Benito, A., Li, Q., Chen, S., Chen, F. F., Yamaoka, S., Verma, I. M., Mak, T. W., Nunez, G. Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-kappa-B induction. J. Biol. Chem. 276: 30589-30597, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CARD10

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Alternative Name: CARD10

Background:

Synonyms: CARD10, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3

Background: Caspase recruitment domain-containing protein 10 is a protein in the CARD-CC protein family that in humans is encoded by the CARD10 gene. The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B through BCL10.

Molecular Weight: 116 kDa

Gene ID: 29775

Pathways: S100 Proteins