CARD10 antibody (AA 450-530)

Catalog No. ABIN7213936

The Rabbit Polyclonal anti-CARD10 antibody is suitable to detect CARD10 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CARD10 antibody (AA 450-530) (ABIN7213936)

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CARD10 Antibody

Binding Specificity: AA 450-530

Purpose: Rabbit Anti-CARD 10 Polyclonal Antibody

Specificity: CARD 10 Polyclonal Antibody detects endogenous levels of CARD 10 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CARD 10 at AA range: 450-530

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CARD10

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Alternative Name: CARD 10

Background: CARD10, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB, see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).

Molecular Weight: 120kD

Gene ID: 29775

UniProt: Q9BWT7

Pathways: S100 Proteins