SOX18 antibody (AA 63-362)

Catalog No. ABIN7602205

The Rabbit Polyclonal anti-SOX18 antibody has been validated for WB, ELISA, ICC, IF and FACS. It is suitable to detect SOX18 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for SOX18 antibody (AA 63-362) (ABIN7602205)

Target: SOX18 (SRY (Sex Determining Region Y)-Box 18 (SOX18))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SOX18 Antibody

Binding Specificity: AA 63-362

Purpose: Anti-SOX18 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SOX18 Antibody Picoband® (ABIN7602205). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SOX18 recombinant protein (Position: E63-E362).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Azuma, T., Seki, N., Yoshikawa, T., Saito, T., Masuho, Y., Muramatsu, M. cDNA cloning, tissue expression, and chromosome mapping of human homolog of SOX18. J. Hum. Genet. 45: 192-195, 2000. 2. Devriendt, K., Vikkula, M., Irrthum, A., Mattjijs, G., Mertens, A., Fryns, J.-P. Autosomal recessive alopecia and lymphedema. (Abstract) Genet. Counsel. 13: 74-75, 2002. 3. Downes, M., Francois, M., Ferguson, C., Parton, R. G., Koopman, P. Vascular defects in the mouse model of hypotrichosis-lymphedema-telangiectasia syndrome indicate a role for SOX18 in blood vessel maturation. Hum. Molec. Genet. 18: 2839-2850, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SOX18

Target: SOX18 (SRY (Sex Determining Region Y)-Box 18 (SOX18))

Alternative Name: SOX18

Background:

Synonyms: Ran-specific GTPase-activating protein,Ran-binding protein 1,RanBP1,RANBP1,

Tissue Specificity: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Background: Transcription factor SOX-18 is a protein that in humans is encoded by the SOX18 gene. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.

Molecular Weight: 41 kDa

Gene ID: 54345

UniProt: P35713