SOX18 antibody (Middle Region)

Catalog No. ABIN2777725

There are 3+ publications for this product available. The Rabbit Polyclonal anti-SOX18 antibody is suitable to detect SOX18 in samples from Human, Rat, Mouse, Cow and Guinea Pig. It has been validated for WB, IHC and IF.

Quick Overview for SOX18 antibody (Middle Region) (ABIN2777725)

Target: SOX18 (SRY (Sex Determining Region Y)-Box 18 (SOX18))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SOX18 Antibody

Binding Specificity: Middle Region

Purpose: SOX18 Antibody - middle region

Sequence: RTAPPAAPLA GLYYGTLGTP GPYPGPLSPP PEAPPLESAE PLGPAADLWA

Predicted Reactivity: Cow: 86%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SOX18. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SOX18

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8112821

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SOX18 antibody (ABIN2777725)

Park, Lee, Park, Choi, Lee, Song, Hwang, Hong, Nakaoka, Makinen, Kim, Alitalo, Hong, Koh: "Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity." in: The Journal of clinical investigation, Vol. 124, Issue 9, pp. 3960-74, (2014) (PubMed).

Deng, Atri, Eichmann, Simons: "Endothelial ERK signaling controls lymphatic fate specification." in: The Journal of clinical investigation, Vol. 123, Issue 3, pp. 1202-15, (2013) (PubMed).

Duong, Proulx, Luciani, Leroux, Detmar, Koopman, Francois: "Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice." in: Cancer research, Vol. 72, Issue 12, pp. 3105-14, (2012) (PubMed).

Target Details for SOX18

Target: SOX18 (SRY (Sex Determining Region Y)-Box 18 (SOX18))

Alternative Name: SOX18

Background:

Background Information: SOX18 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.

Gene Name: SRY (sex determining region Y)-box 18

Alternative Symbols: HLTS, HLTRS

Protein Name: Transcription factor SOX-18

Molecular Weight: 41kDa

Gene ID: 54345

NCBI Accession: NP_060889

UniProt: P35713