TCF12 antibody (AA 67-300)

Catalog No. ABIN7602265

The Rabbit Polyclonal anti-TCF12 antibody has been validated for WB, ELISA and FACS. It is suitable to detect TCF12 in samples from Human, Rat and Mouse.

Quick Overview for TCF12 antibody (AA 67-300) (ABIN7602265)

Target: TCF12 (Transcription Factor 12 (TCF12))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TCF12 Antibody

Binding Specificity: AA 67-300

Purpose: Anti-HEB/TCF12 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HEB/TCF12 Antibody Picoband® (ABIN7602265). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HEB/TCF12 recombinant protein (Position: S67-G300). Human TCF12 shares 96.6% amino acid (aa) sequence identity with both mouse and rat TCF12.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Davis, E. E., Balasubramanian, R., Kupchinsky, Z. A., Keefe, D. L., Jr., Plummer, L., Khan, K., Meczekalski, B., Heath, K. E., Lopez-Gonzalez, V., Ballesta-Martinez, M. J., Margabanthu, G., Price, S., and 12 others. TCF12 haploinsufficiency causes autosomal dominant Kallmann syndrome and reveals network-level interactions between causal loci. Hum. Molec. Genet. 29: 2435-2450, 2020. 2. Doyle, K., Zhang, Y., Baer, R., Bina, M. Distinguishable patterns of protein-DNA interactions involving complexes of basic helix-loop-helix proteins. J. Biol. Chem. 269: 12099-12105, 1994. 3. Gan, T.-I., Rowen, L., Nesbitt, R., Roe, B. A., Wu, H., Hu, P., Yao, Z., Kim, U.-J., O'Sickey, T., Bina, M. Genomic organization of human TCF12 gene and spliced mRNA variants producing isoforms of transcription factor HTF4. Cytogenet. Genome Res. 98: 245-248, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TCF12

Target: TCF12 (Transcription Factor 12 (TCF12))

Alternative Name: TCF12

Background:

Synonyms: TCF12, BHLHB20, HEB, HTF4, Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4

Background: Transcription factor 12 is a protein that in humans is encoded by the TCF12 gene. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Molecular Weight: 85 kDa

Gene ID: 6938

UniProt: Q99081

Pathways: SARS-CoV-2 Protein Interactome