TCF12 antibody (AA 67-300)

Catalog No. ABIN7602265

The Rabbit Polyclonal anti-TCF12 antibody is suitable to detect TCF12 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA and FACS.

Quick Overview for TCF12 antibody (AA 67-300) (ABIN7602265)

Target: TCF12 (Transcription Factor 12 (TCF12))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TCF12 Antibody

Binding Specificity: AA 67-300

Purpose: Anti-HEB/TCF12 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HEB/TCF12 Antibody (ABIN7602265). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HEB/TCF12 recombinant protein (Position: S67-G300). Human TCF12 shares 96.6% amino acid (aa) sequence identity with both mouse and rat TCF12.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TCF12

Target: TCF12 (Transcription Factor 12 (TCF12))

Alternative Name: TCF12

Background:

Synonyms: TCF12, BHLHB20, HEB, HTF4, Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4

Background: Transcription factor 12 is a protein that in humans is encoded by the TCF12 gene. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Molecular Weight: 85 kDa

Gene ID: 6938

UniProt: Q99081

Pathways: SARS-CoV-2 Protein Interactome