ATG12 antibody (AA 68-99)

Catalog No. ABIN7602293

This anti-ATG12 antibody is a Rabbit Polyclonal antibody detecting ATG12 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for ATG12 antibody (AA 68-99) (ABIN7602293)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ATG12 Antibody

Binding Specificity: AA 68-99

Purpose: Anti-ATG12 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ATG12 Antibody Picoband® (ABIN7602293). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATG12 recombinant protein (Position: M68-Q99).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mizushima, N., Sugita, H., Yoshimori, T., Ohsumi, Y. A new protein conjugation system in human: the counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273: 33889-33892, 1998. 2. Tanida, I., Tanida-Miyake, E., Komatsu, M., Ueno, T., Kominami, E. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J. Biol. Chem. 277: 13739-13744, 2002. 3. Ueno, K., Kumagai, T., Kijima, T., Kishimoto, T., Hosoe, S. Cloning and tissue expression of cDNAs from chromosome 5q21-22 which is frequently deleted in advanced lung cancer. Hum. Genet. 102: 63-68, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12

Background:

Synonyms: Fibrinogen alpha chain, FGA

Tissue Specificity: Detected in blood plasma (at protein level).

Background: Autophagy related 12 is a protein that in humans is encoded by the ATG12 gene. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

Molecular Weight: 48 kDa

Gene ID: 9140

UniProt: O94817

Pathways: Autophagy