Ribonuclease H1 antibody (AA 7-223)

Catalog No. ABIN7602317

This anti-Ribonuclease H1 antibody is a Rabbit Polyclonal antibody detecting Ribonuclease H1 in WB, ELISA, ICC, IF and FACS. Suitable for Human and Mouse.

Quick Overview for Ribonuclease H1 antibody (AA 7-223) (ABIN7602317)

Target: Ribonuclease H1 (RNASEH1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ribonuclease H1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Ribonuclease H1 Antibody

Binding Specificity: AA 7-223

Purpose: Anti-RNASEH1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RNASEH1 Antibody Picoband® (ABIN7602317). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNASEH1 recombinant protein (Position: L7-Q223).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cerritelli, S. M., Crouch, R. J. Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase H1. Genomics 53: 300-307, 1998. 2. Cerritelli, S. M., Frolova, E. G., Feng, C., Grinberg, A., Love, P. E., Crouch, R. J. Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice. Molec. Cell 11: 807-815, 2003. 3. Nowotny, M., Gaidamakov, S. A., Ghirlando, R., Cerritelli, S. M., Crouch, R. J., Yang, W. Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. Molec. Cell 28: 264-276, 2007. Note: Erratum: Molec. Cell 28: 513 only, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Ribonuclease H1

Target: Ribonuclease H1 (RNASEH1)

Alternative Name: RNASEH1

Background:

Synonyms: Spindle and kinetochore-associated protein 2, Protein FAM33A, SKA2, FAM33A

Background: Ribonuclease H1 also known as RNase H1 is an enzyme that in humans is encoded by the RNASEH1 gene. This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are ed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17.

Molecular Weight: 38 kDa

Gene ID: 246243

UniProt: O60930