HDAC9 antibody (AA 7-469)

Catalog No. ABIN7602333

This Rabbit Polyclonal antibody specifically detects HDAC9 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for HDAC9 antibody (AA 7-469) (ABIN7602333)

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-HDAC9 Antibody

Binding Specificity: AA 7-469

Purpose: Anti-HDAC9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HDAC9 Antibody Picoband® (ABIN7602333). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HDAC9 recombinant protein (Position: S7-Q469).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mejat, A., Ramond, F., Bassel-Duby, R., Khochbin, S., Olson, E. N., Schaeffer, L. Histone deacetylase 9 couples neuronal activity to muscle chromatin acetylation and gene expression. Nature Neurosci. 8: 313-321, 2005. 2. Sparrow, D. B., Miska, E. A., Langley, E., Reynaud-Deonauth, S., Kotecha, S., Towers, N., Spohr, G., Kouzarides, T., Mohun, T. J. MEF-2 function is modified by a novel co-repressor, MITR. EMBO J. 18: 5085-5098, 1999. 3. Zhou, X., Marks, P. A., Rifkind, R. A., Richon, V. M. Cloning and characterization of a histone deacetylase, HDAC9. Proc. Nat. Acad. Sci. 98: 10572-10577, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: HDAC9

Background:

Synonyms: Probetacellulin, Betacellulin, BTC, BTC

Tissue Specificity: Highly expressed in testis.

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.

Molecular Weight: 150 kDa

Gene ID: 9734

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development