TRMT61B antibody (AA 7-477)

Catalog No. ABIN7602334

The Rabbit Polyclonal anti-TRMT61B antibody has been validated for WB, ELISA, IHC, FACS and IF. It is suitable to detect TRMT61B in samples from Human.

Quick Overview for TRMT61B antibody (AA 7-477) (ABIN7602334)

Target: TRMT61B (tRNA Methyltransferase 61 (TRMT61B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRMT61B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-TRMT61B Antibody

Binding Specificity: AA 7-477

Purpose: Anti-TRMT61B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRMT61B Antibody Picoband® (ABIN7602334). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRMT61B recombinant protein (Position: R7-N477).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bar-Yaacov, D., Frumkin, I., Yashiro, I., Chujo, T., Ishigami, Y., Chemla, Y., Blumberg, A., Schlesinger, O., Bieri, P., Greber, B., Ban, N., Zarivach, R., Alfonta, L., Pilpel, Y., Suzuki, T., Mishmar, D. Mitochondrial 16S rRNA is methylated by tRNA methyltransferase TRMT61B in all vertebrates. PLoS Biol. 14: e1002557, 2016. Note: Erratum: PLoS Biol. 15: e1002594, 2017. 2. Chujo, T., Suzuki, T. Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs. RNA 18: 2269-2276, 2012. 3. Gross, M. B. Personal Communication. Baltimore, Md. 6/24/2021.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRMT61B

Target: TRMT61B (tRNA Methyltransferase 61 (TRMT61B))

Alternative Name: TRMT61B

Background:

Synonyms: Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FJ

Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Background: Enables mRNA (adenine-N1-)-methyltransferase activity, rRNA (adenine) methyltransferase activity, and tRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation, mitochondrial tRNA methylation, and protein homooligomerization. Located in mitochondrion.

Molecular Weight: 57 kDa

Gene ID: 55006