LDLR antibody (AA 705-860)

Catalog No. ABIN7602357

This Rabbit Polyclonal antibody specifically detects LDLR in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for LDLR antibody (AA 705-860) (ABIN7602357)

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDLR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-LDLR Antibody

Binding Specificity: AA 705-860

Purpose: Anti-LDLR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-LDLR Antibody Picoband® (ABIN7602357). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LDLR recombinant protein (Position: A705-A860).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aalto-Setala, K., Helve, E., Kovanen, P. T., Kontula, K. Finnish type of low absorbance lipoprotein receptor gene mutation (FH-Helsinki) deletes exons encoding the carboxy-terminal part of the receptor and creates an internalization-defective phenotype. J. Clin. Invest. 84: 499-505, 1989. 2. Aalto-Setala, K., Koivisto, U.-M., Miettinen, T. A., Gylling, H., Kesaniemi, Y. A., Savolainen, M., Pyorala, K., Ebeling, T., Mononen, I., Turtola, H., Viikari, J., Kontula, K. Prevalence and geographical distribution of major LDL receptor gene rearrangements in Finland. J. Intern. Med. 231: 227-234, 1992. 3. Agnello, V., Abel, G., Elfahal, M., Knight, G. B., Zhang, Q.-X. Hepatitis C virus and other flaviviridae viruses enter cells via low absorbance lipoprotein receptor. Proc. Nat. Acad. Sci. 96: 12766-12771, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LDLR

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Alternative Name: LDLR

Background:

Synonyms: Low-density lipoprotein receptor, LDL receptor, LDLR

Tissue Specificity: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.

Background: In humans, the LDL receptor protein is encoded by the LDLR gene on chromosome 19. It is mapped to 19p13.2. The low absorbance lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low absorbance lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

Molecular Weight: 140-160 kDa

Gene ID: 3949

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism