NEK11 antibody (AA 72-630)

Catalog No. ABIN7602376

The Rabbit Polyclonal anti-NEK11 antibody has been validated for WB and ELISA. It is suitable to detect NEK11 in samples from Human, Mouse and Rat.

Quick Overview for NEK11 antibody (AA 72-630) (ABIN7602376)

Target: NEK11 (NIMA (Never In Mitosis Gene A)-Related Kinase 11 (NEK11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NEK11 Antibody

Binding Specificity: AA 72-630

Purpose: Anti-NEK11 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NEK11 Antibody Picoband® (ABIN7602376). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NEK11 recombinant protein (Position: E72-Q630). Human NEK11 shares 71.1% amino acid (aa) sequence identity with mouse NEK11.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, Human
1. Noguchi, K., Fukazawa, H., Murakami, Y., Uehara, Y. Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses. J. Biol. Chem. 277: 39655-39665, 2002. 2. Noguchi, K., Fukazawa, H., Murakami, Y., Uehara, Y. Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells. J. Biol. Chem. 279: 32716-32727, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NEK11

Target: NEK11 (NIMA (Never In Mitosis Gene A)-Related Kinase 11 (NEK11))

Alternative Name: NEK11

Background:

Synonyms: NEK11, Serine/threonine-protein kinase Nek11, EC 2.7.11.1, Never in mitosis A-related kinase 11, NimA-related protein kinase 11

Background: This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.

Molecular Weight: 74 kDa

Gene ID: 79858