PRPF31 antibody (AA 73-488)

Catalog No. ABIN7602388

The Rabbit Polyclonal anti-PRPF31 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect PRPF31 in samples from Human, Mouse and Rat.

Quick Overview for PRPF31 antibody (AA 73-488) (ABIN7602388)

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRPF31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PRPF31 Antibody

Binding Specificity: AA 73-488

Purpose: Anti-PRPF31 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRPF31 Antibody Picoband® (ABIN7602388). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRPF31 recombinant protein (Position: K73-K488).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Al-Maghtheh, M., Vithana, E., Tarttelin, E., Jay, M., Evans, K., Moore, T., Bhattacharya, S., Inglehearn, C. F. Evidence for a major retinitis pigmentosa locus on 19q13.4 (RP11), and association with a unique bimodal expressivity phenotype. Am. J. Hum. Genet. 59: 864-871, 1996. 2. Berson, E. L., Gouras, P., Gunkel, R. D., Myrianthopoulos, N. C. Dominant retinitis pigmentosa with reduced penetrance. Arch. Ophthal. 81: 226-234, 1969. 3. Deery, E. C., Vithana, E. N., Newbold, R. J., Gallon, V. A., Bhattacharya, S. S., Warren, M. J., Hunt, D. M., Wilkie, S. E. Disease mechanism for retinitis pigmentosa (RP11) caused by mutations in the splicing factor gene PRPF31. Hum. Molec. Genet. 11: 3209-3219, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRPF31

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Alternative Name: PRPF31

Background:

Synonyms: Tumor necrosis factor receptor superfamily member 18, Glucocorticoid-induced TNFR-related protein, CD357, Tnfrsf18, Gitr

Tissue Specificity: Detected in thymus and in activated splenocytes.

Background: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), also known as PRPF31, is a protein which in humans is encoded by the PRPF31 gene. This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.

Molecular Weight: 60 kDa

Gene ID: 26121

Pathways: Ribonucleoprotein Complex Subunit Organization