NOL10 antibody (AA 73-497)

Catalog No. ABIN7602390

This Rabbit Polyclonal antibody specifically detects NOL10 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for NOL10 antibody (AA 73-497) (ABIN7602390)

Target: NOL10 (Nucleolar Protein 10 (NOL10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOL10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NOL10 Antibody

Binding Specificity: AA 73-497

Purpose: Anti-NOL10 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NOL10 Antibody Picoband® (ABIN7602390). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOL10 recombinant protein (Position: Y73-D497).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/20/2015. 2. Wada, K., Sato, M., Araki, N., Kumeta, M., Hirai, Y., Takeyasu, K., Furukawa, K., Horigome, T. Dynamics of WD-repeat containing proteins in SSU processome components. Biochem. Cell Biol. 92: 191-199, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NOL10

Target: NOL10 (Nucleolar Protein 10 (NOL10))

Alternative Name: NOL10

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: By immunoprecipitation of human AATF we discovered that AATF forms a salt-stable protein complex together with neuroguidin (NGDN) and NOL10, and demonstrate that the AATF-NGDN-NOL10 (ANN) complex functions in ribosome biogenesis. All three ANN complex members localize to nucleoli and display a mutual dependence with respect to protein stability. Mapping of protein-protein interaction domains revealed the importance of both the evolutionary conserved WD40 repeats in NOL10 and the UTP3/SAS10 domain in NGDN for complex formation.

Molecular Weight: 80 kDa

Gene ID: 79954

Pathways: SARS-CoV-2 Protein Interactome